Meso Scale Discovery assays are a robust technology for measuring analytes in complex biological matrices. Assay options such as MSD PK assay and MSD cytokine multiplex assay help researchers detect and characterize biomarkers such as intercellular signaling proteins and cytokines that directly impact drug discovery and development projects. Meso Scale Discovery multiplex assay combines electrochemiluminescence detection and multiarray technology to provide excellent sensitivity and robust multiplexing capacities in a unique detection system. However, like developing bioanalytical systems such as LC-MS/MS method validation, MSD assay will need similar yet robust development approaches.
Multiplex Meso Scale pharmacokinetics methods can detect and quantify multiple analytes in a single sample volume. Multiplexing capacities reduce time and resources and is an ideal solution for analyzing limited volumes of samples. . The current article discusses the benefits of multiplexing in PK MesoScale assay.
Advantages of Multiplex PK MesoScale Assay
The last two or three decades have witnessed an exponential increase in the number of biological therapeutic proteins introduced in the clinical space. Several complex biomolecular modalities are being developed to improve these biological therapeutics. Multiplex Meso Scale PK assay can help develop biologic therapeutic proteins. Biological molecules are large and complex. Researches require multiple data points for fully comprehending the PK profile of a biologic drug product. Multiplex PK assays can evaluate and monitor numerous analytes in a single assay sample and provide a comprehensive depiction of PK parameters from initial first-in-human studies through subsequent clinical trial phases.
Multiplexing approaches are generally employed for detecting protein biomarkers. Bioanalytical multiplex immunoassay detection reagents are conjugated with fluorescent tags. However, these fluorescent tags may generate interference and potentially inaccurate data. Meso Scale Discovery U-plex assay, provides an alternative to eliminate the use of different fluorescent tags. MSD instruments have up to 10 microspots inside a single well to physically separate individual analytes per microspot.
Also Read: The Importance of Quality Control in Bioanalytical Testing Laboratories
Generally, pharmacokinetic testing methods need a single readout platform to analyze a single drug form. However, multiplex Meso Scale PK assay can dramatically increase the understanding of the product by estimating the total PK and free PK data along with its binding characteristics. Not to mention using multiplex PK assay help in saving biological samples. Biological samples from non-human and animal models are often low in volume and are subjected to additional processing. This approach further affects the molecule. Besides, blood collection initiatives have specific restrictions affecting the collected sample volume from the target clinical population. Suppose researchers can access a robust bioanalytical method such as multiplex Meso Scale PK assay; they can quantify multiple analytes in a single assay volume and eliminate the variability cost due to additional sampling.
What is Next for Meso Scale Discovery Multiplex Assays?
Meso Scale Discovery multiplex assay can increase the efficiency and throughput of bioanalytical laboratory testing. It is an ideal platform for conducting preclinical and clinical bioanalysis for pharmaceutical drug forms. Sponsors can implement research-use kits immediately while qualifying and validating MSD assays simultaneously to meet subsequent regulatory guidelines.
